STAINING, PRACTICAL AND THEORETICAL 



CHROME HAEMATOXYLIN (HANSEN) 



Haematoxylin I % aqueous .. .. 30 ml. 



Chrome alum 10% aqueous . . • • 30 ml. 



Potassium dichromate 10% aqueous . . 2 ml. 



Sulphuric acid, cone. . . . . . . 0-2 ml. 



CHROME HAEMATOXYLIN (SCHWEITZER) 



Haematoxylin 1% aqueous 

 Chrome alum 10% aqueous 

 Potassium dichromate 10% aqueous 

 Distilled water 

 Sulphuric acid, cone. 



COTTON BLUE - LACTOPHENOL 



Cotton blue 

 Lactophenol . . 



50 ml. 

 50 ml. 

 275 ml. 

 22-25 ™'- 

 4 ml. 



I gm. 

 100 ml. 



COTTON BLUE - MAGENTA LACTOPHENOL 



Cotton blue i % in lactophenol . . . . 8 ml. 



Magenta 1% in lactophenol . . . . 100 ml. 



Lactophenol . . . . . . . . 100 ml. 



FAST-GREEN FCF IN CELLOSOLVE 



Cellosolve . . , . . . . . . . 100 ml. 



Fast-green FCF . . . . . . • • 3 gm. 



Heat in a flask on a water bath for about half an hour with occasional 

 stirring; then cool and filter. 



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