HAEMATOXYLIN (DELAFIELD) 



Haematoxylin 3*5% in absolute alcohol . . 100 ml. 

 Ammonia alum 6-25% aqueous . . . . 320 ml. 



Glycerine . . . . . . . . . . 80 ml. 



Mix thoroughly and allow to ripen for at least three months; then 

 filter before use. 



HAEMATOXYLIN (EHRLICH) 



Haematoxylin 2% in absolute alcohol . . 100 ml. 



Potash alum 2-5% aqueous .. .. 100 ml. 



Glycerine . . . . . . . . . . 100 ml. 



Glacial acetic acid . . . . . . . . 10 ml. 



Mix well; allow to ripen in a 500-ml. closed bottle for three months 

 or: 



Dissolve I gm. Ehrlich haematoxylin powder (Michrome No. '370) in a 

 mixture of 4 ml. glacial acetic acid, 80 ml. 50% alcohol and 40 ml. 

 glycerine; then filter. The solution requires no ripening and is ready 

 for immediate use. 



HAEMATOXYLIN (HANSEN) 



Iron alum 10% aqueous . . . . . . 45 ml. 



Haematoxylin 10% in absolute alcohol . . 7-5 ml. 

 Distilled water . . . . . . . . 47-5 ml. 



Mix well; boil; then filter and make the volume up to 100 ml. with 

 distilled water. 



HAEMATOXYLIN (HARRIS) 



Haematoxylin 10% in absolute alcohol 



Mercuric oxide 



Potash alum 10% aqueous 



Glacial acetic acid . . 



Mix the haematoxylin and alum solutions: raise to boiling point, 

 then add the mercuric oxide and when the solution turns deep purple 

 turn off the heat : then cool and add the acetic acid. 



5 ml. 

 0-25 gm. 

 100 ml. 

 4 ml. 



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