STAINING, PRACTICAL AND THEORETICAL 



HAEMATOXYLIN (HEIDENHAIN) 



No. I 



Iron alum 4 gm. 



Distilled water . 100 ml. 



Dissolve by shaking. Do not heat. 

 No. 2 



Haematoxylin 10% in absolute alcohol 



(ripened three to six months) . . . . 5 ml. 



Distilled water 95 ml. 



HAEMATOXYLIN (IRON) (ANDERSON) 



No. I 



Haematoxylin 10% in absolute alcohol . . 5 ml. 



Absolute alcohol . . . . . . • • 45 ml. 



Distilled water 50 ml. 



Calcium hypochlorite 2% aqueous . . 3 ml. 



No. 2 



Iron alum 4% aqueous 100 ml. 



Sulphuric acid cone. . . . . • • 3 ml. 



For use, mix two volumes of solution No. i with one volume of No. 

 2; this mixture keeps for a few hours only, and should therefore be 

 prepared only when needed for immediate use. 



HAEMATOXYLIN (JANSSEN) 



Iron alum 10% aqueous . . 

 Haematoxylin 10% in absolute alcohol 

 Methyl alcohol, absolute . . 

 Glycerine 

 Distilled water 



Allow the mixture to stand for a week before use 



50 ml. 

 10 ml. 

 15 ml. 

 15 ml. 

 20 ml. 



HAEMATOXYLIN KULTSCHITZKY 



Haematoxylin 10% alcoholic . . . . 10 ml. 



Acetic acid 2% aqueous . . . . . . 100 ml. 



Dissolve the haematoxylin in the alcohol then shake thoroughly with 

 the acetic acid solution. 



HAEMATOXYLIN (MARTINOTTI) 



Ammonia alum 10% aqueous . . . . 15 ml. 

 Methyl alcohol, absolute . . . . . . 15 ml. 



556 



