STAINING, PRACTICAL AND THEORETICAL 



Glacial acetic acid . . 

 Sodium or potassium iodate 



3 ml. 

 I gm. 



HAEMATOXYLIN (SCHRODER) 



Lithium carbonate 1% aqueous 

 Haematoxylin 1% aqueous 

 Distilled water 



4 ml. 

 30 ml. 

 66 ml. 



HAEMATOXYLIN (SQUIRE) 



Haematoxylin 10% in absolute alcohol . . 10 ml. 



Ammonia alum .. .. .. .. i-2gm. 



Calcium chloride . . . . . . . . 8 gm. 



Distilled water . . . . . . . . 10-5 ml. 



Absolute alcohol . . . . . . . . 96 ml. 



Dissolve the calcium chloride in the water; then shake in the am- 

 monia alum and mix the solution with the haematoxylin and the 

 alcohol. 



Dissolve the haematoxylin in the dioxane; dissolve the phospho- 

 molybdic acid in the water and the ethylene glycol; mix the two 

 solutions; then add the hydrogen peroxide and mix thoroughly. 



HAEMATOXYLIN (WEIGERT) 



A. Haematoxylin 10% in absolute alcohol 



(ripened three to six months) . . 

 Absolute alcohol 



B. Ferric chloride (hydrated) 30% aqueous 

 Hydrochloric acid . . 

 Distilled water 



10 ml. 

 90 ml, 



4 ml. 

 I ml. 

 95 ml. 



HITCHCOCK & EHRICH STAIN 



Malachite Green 2% aqueous 

 Acridin red 2% aqueous . . 



558 



15 ml- 

 45 ml. 



