STAINING, PRACTICAL AND THEORETICAL 



MAY-GRUNWALD STAIN 



May-Grunwald stain powder 

 Pure methyl alcohol 



Dissolve by heating in a flask, lightly plugged with cotton-wool, on a 

 water bath. Allow to cool, then filter. 



0-25 gm. 

 ICO ml. 



METHYL GREEN - PYRONIN (PAPPENHEIM - UNNA) 



Methyl green .. .. .. ..0-15 gm. 



Pyronin (Y or G) 



Phenol 0*5% aqueous solution 



Glycerine 



Absolute alcohol 



Dissolve the powder in the 

 with the glycerine and alcohol 



0-25 gm. 

 80 ml. 

 20 ml. 

 2-5 ml. 



phenol solution; then shake vigorously 

 warm to dissolve ; then filter. 



METHYL VIOLET (JENSEN) 



Methyl violet 6B . . 

 Distilled water 



0-5 gm. 

 100 ml. 



METHYLENE BLUE, AQUEOUS 



Methylene blue 

 Distilled water 



METHYLENE BLUE (BORAX) 



(MANSON) 



Methylene blue 

 Borax 5 % aqueous . . 



Note: Do not heat above 60° C. 



I gm. 

 100 ml. 



2gm. 

 100 ml. 



METHYLENE BLUE (BORAX) 



(UNNA) 



Methylene blue 

 Borax i % aqueous 



Note: Do not heat borax methylene blue solutions over 60° C. 



I gm. 

 100 ml. 



METHYLENE BLUE (LOEFFLER) 



Methylene blue 



Absolute alcohol 



Potass, hydroxide o-oi % aqueous 



3 gm- 

 30 ml. 



100 ml. 



METHYLENE BLUE, POLYCHROME (UNNA) 



Methylene blue . . . . . . . . i gm. 



560 



