SECTION THREE 



Potass, carbonate . . . . , . . . i gm. 



Absolute alcohol . . . . . . . , 20 ml. 



Water . . . . . . . . . . 100 ml. 



Heat to 60° C. for fifteen minutes. Cool and filter. 



or: 



Polychrome methylene blue (Michrome 



No. 1024). . . . . . . . • • 3 gm. 



Distilled water . . . . . . . . 200 gm. 



Absolute alcohol . . . . . . . . 10 ml. 



Dissolve by heating to 6o°C. and maintain at that temperature for 

 fifteen minutes. Cool and filter; then add the alcohol and mix 

 thoroughly. 



METHYLENE BLUE SULPHURIC (GABBET) 



Distilled water . . . . . . • • 75 ml- 



Sulphuric acid, cone. . . . . • • 25 ml. 



Methylene blue . . . . . . . . 2 gm. 



Pour the sulphuric acid into the water carefully, in a flask, then add 

 the methylene blue and shake or stir to dissolve. 



MUCICARMINE (MAYER) 



Stock solution: 



Carmine . . . . . . . . . . i gm. 



Aluminium chloride, anhydrous . . . . 0-5 gm. 



Mix thoroughly by grinding to a homogeneous powder in a mortar; 

 then place the mixture in a flask with 100 ml. of 50% alcohol and boil 

 for two and a half to five minutes on a water bath, shaking the flask 

 at intervals. Cool under the tap at once and filter when cold. 



For use, dilute one volume of this solution with nine volumes distilled 

 water; 



or: 



Shake 5 gm. of Mucicarmine powder (Michrome No. 356) with 200 

 ml. of 50% alcohol; then heat to boiling point and continue boiHng for 

 2^ minutes. Cool; filter. 



MUCI HAEMATEIN, ALCOHOLIC 



Haematein 0-2% in 70% alcohol . . . . 100 ml. 



Nitric acid .. .. .. ,. .. o-i ml. 



Aluminium chloride . . . . . . o-i gm. 



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