STAINING, PRACTICAL AND THEORETICAL 



MUCI HAEMATEIN, AQUEOUS 



Haematein 0-5% aqueous . . . . . . 40 ml. 



Aluminium chloride 0-5%. . . . . . 20 ml. 



Glycerine . . . . . . . . . . 40 ml. 



Dissolve by warming and shaking ; then cool and filter. 



NEISSER'S STAIN 



A. Methylene blue 

 Absolute alcohol 

 Glacial acetic acid 

 Distilled water 



B. Bismarck brown 

 Distilled water 



NEUTRAL RED (JENSEN) 



Neutral red . . 

 I % acetic acid 

 Distilled water 



0-2 gm. 

 4 ml. 

 10 ml. 

 190 ml. 



0-2 gm. 

 100 ml. 



O'l gm. 

 o*2 ml. 

 100 ml. 



NISSL'S METHYLENE BLUE 



Venetian soap .. .. .. .. 0-175 gm. 



Distilled water . . . . . . . . 80 ml. 



Dissolve; then add: 



Methylene Blue 2% aqueous . . . . 20 ml. 



Shake vigorously then store in a well-closed bottle for at least four 

 months, before use, shaking once or twice a week during this period. 



ORANGE FUCHSIN (SQUIRE) 



Acid fuchsin 10% aqueous 

 Orange G 3% aqueous 

 Distilled water 

 Absolute alcohol 



ID ml. 

 200 ml. 

 30 ml. 

 60 ml. 



ORANGE G, AQUEOUS 



Orange G . . 

 Distilled water 



ORCEIN (UNNA - TANZER) 



Orcein 

 70% Alcohol 

 Hydrochloric acid, cone. 



I gm. 

 100 ml. 



I gm. 

 100 ml. 

 I ml. 



562 



