SECTION THREE 



PARACARMINE (MAYER) 



Carminic acid 



Aluminium chloride, hydrated 

 Calcium chloride, anhydrous 

 Alcohol 70% 



PERIODIC ACID - SCHIFF 



(After Ritter & Oleson, 1950) 



A. Periodic acid 



Distilled water 

 M/5 sodium acetate* 



I gm. 

 0-5 gm. 

 4gm. 

 100 ml. 



4gm. 

 100 ml. 

 50 ml. 



B. Reducing rinse 



Potassium iodide 



Sodium thiosulphate, pentahydrate 



Distilled water 



Dissolve, then add: 

 Absolute alcohol 



followed by: 

 Hydrochloric acid 2N 



igm. 

 igm. 

 20 ml. 



30 ml. 



0-5 ml. 



C. Fuchsin - Sulphite 



Basic fuchsin 

 Distilled water 

 Hydrochloric acid 2N 

 Potassium metabisulphite 

 Decolorizing charcoal 



. . I gm. 



200 ml. 

 . . 5 ml. 

 . . 2 gm. 

 about I gm. 



First boil the distilled water; then cool to about 50° C. and dissolve 

 the basic fuchsin in it, followed by the potassium metabisulphite. Add 

 the hydrochloric acid; plug the neck of the flask with cotton wool 

 before shaking. Leave the flask to stand overnight; then add the char- 

 coal, shake well, then filter. 



D. Sulphite rinse water 



Distilled water 

 Potassium metabisulphite 

 Hydrochloric acid, cone. 



100 ml. 

 0-4 gm. 

 I ml. 



* Prepared by dissolving 0-193 gi"- crystalline sodium acetate in 50 ml. of 

 absolute (ethyl) alcohol. 



