STAINING, PRACTICAL AND THEORETICAL 



PHLOXIN - PROPYLENE GLYCOL 



(For eosinophile counts) 



Phloxin 0*1% in distilled water . . • • 50 ml. 



Propylene glycol . . . . , . • • 50 ml. 



This reagent is stated to give more consistent results with no destruc- 

 tion of eosinophiles. 



Reference: Henneman, Waxier & Westenhaver (1949). 



PICRO ANILINE BLUE, ALCOHOLIC 



Aniline blue alcohol soluble . . . . i gm. 



70% alcohol. . .. .. .. .. 50 ml. 



Picric acid, 1% in 70% alcohol . . • • 50 ml. 



PICRO ANILINE BLUE, AQUEOUS 



Aniline blue water soluble . . . . o-i gm. 



Picric acid saturated, aqueous . . . . loo ml. 



PICROCARMINE 



Picrocarmine powder (Mi chrome No. 1087) ^ §"!• 

 Distilled water . . . . . . • • 5° nil. 



Boil gently for five minutes: allow to cool; then filter and make up 

 the volume to 50 ml. with distilled water. 



PICRO INDIGO CARMINE 



Picric acid, saturated aqueous . . . . 100 ml. 



Indigo carmine . . . . . . . . 0-25 gm. 



RANDOLPH'S BLOOD COUNTING FLUID 



Solution A 



Methylene blue . . . . . . . . o-i gm. 



Propylene glycol . . . . . . . , loo ml. 



Solution B 



Eosin, bluish . . . . . . . . o-i gm. 



Distilled water . . . . . . . . 100 ml. 



Mix equal volumes of the two solutions and use as a diluting fluid 

 in the white cell pipette. The mixture of the blood and the diluting 

 fluid should remain in the pipette for fifteen minutes before filling the 

 counting chamber. 



Reference: Randolph, T. G. (1943). 



