SECTION THREE 



RED CELL COUNTING FLUID (O. T. George, 1952) 



Sodium citrate 3% aqueous . . . . 100 ml. 



Formalin (Formaldehyde 40%) . . • • i ml. 



Eosin, bluish . . . . . . . . o-6 gm. 



This solution stains the erythrocytes and causes the cells to lie flat 

 in a single focal plane, thereby facilitating enumeration. 



SAFRANIN 



Safranin 



Distilled water or 70% alcohol 



I gm. 

 100 ml. 



SCARLET R, ALKALINE (HERXHEIMER) 



Absolute alcohol 35 "^1- 



Distilled water 5 ml. 



Sodium hydroxide 10% aqueous . . . . 10 ml. 



Scarlet R . . . . . . . . . . i gm. 



Place in a flask and plug the neck lightly with cotton wool. Heat on 



a hot water bath until a considerable portion of the dye has gone into 

 solution ; then allow to cool before filtering. 



SHORR'S STAIN 



A. Harris haematoxylin. 



B. Biebrich scarlet . . . . . . . . i gm. 



Orange G . . . . . . . . . . 0-4 gm. 



Distilled water . . . . . . • • 99 ml. 



Glacial acetic acid . . . . . . . . i ml. 



Heat the water: add the stains; stir until dissolved; allow to cool; 

 then add acetic acid ; shake well and filter. 



C. Phosphotungstic acid 10% . . • • 25 ml. 

 Phosphomolybdic acid 10% . . . . 25 ml. 

 Distilled water 100 ml. 



D. Fast green FCF aqueous 5 % . . • • 5 ml. 



Acetic acid i % 32 ml. 



Distilled water 64 ml. 



SHORR'S STAIN 

 (Single solution) 



Biebrich scarlet, aqueous . . . . . . 0-5 gm. 



Orange G . . . . . . . . . . 0-25 gm. 



Fast green FCF . . . . . . . . 0-075 g"^- 



Phosphotungstic acid . . . . . . 0-5 gm. 



565 



