STAINING, PRACTICAL AND THEORETICAL 



Phosphomolybdic acid . . . . . . 0-5 gm. 



Glacial acetic acid . . . . . . . . i ml. 



Alcohol 50% 100 ml. 



Dissolve by warming; then allow to cool and filter. 



or: 



Shorr's Stain powder (Michrome No. 521) i gm. 



Alcohol 50% 55 ml. 



Glacial acetic acid . . . . . . . . 0*5 ml. 



Dissolve the stain by warming with the alcohol; allow to cool; then 

 add the acetic acid, shake well and filter. 



TOLUIDINE BLUE (PONDER'S STAIN) FOR DIPHTHERIA 



Toluidine blue 0-02% aqueous .. .. 100 ml. 



Glacial acetic acid . . 

 Alcohol 



TOLUIDINE BLUE (PUGH) 



Toluidine blue 

 Glacial acetic acid . . 

 Absolute alcohol 

 Distilled water 



I ml. 

 5 ml. 



0-2 gm. 



5 ml- 

 2 ml. 



43 ml. 



WRIGHT'S STAIN 



Wright's stain powder 

 Methyl alcohol, pure 



Dissolve by heating in a flask, lightly plugged with cotton wool, on 

 a water bath. Allow to cool; then filter 



0-3 gm. 

 100 ml. 



or. 



Wright's stain (Michrome) . . . . i tablet 



Methyl alcohol, pure 33 ml. 



Crush the tablet; then dissolve by shaking or heating on a water bath. 



REFRACTIVE INDICES 



Absolute alcohol (ethyl) 



Acetic acid, glacial 



Acetone . . 



Aniline oil 



Aniseed oil 



Aquamount 



Balsam tolu 



1-361 

 1-371 



1-359 

 1-586 



1-557 



1-435 

 1-640 



566 



