STAINING, PRACTICAL AND THEORETICAL 



7. Rinse in distilled water; drain and carefully blot away excess 

 water. 



8. Dehydrate and differentiate the Borrel Blue, controlling by 

 examination under the microscope. 



9. Clear in xylol and mount. 



Results: 



T.B. or leprosy, bright red; other bacteria, blue ; cells and cell 

 debris, varying shades of blue; cell nuclei, blue. 



N.B.: For demonstrating leprosy, differentiation of the carbol 

 fuchsin (stages 4 and 5) must be very carefully carried out, as this 

 organism is more easily completely decolorized than T.B. 



References : 

 Carleton, H. M. & Leach, E. H. (i947), PP- 320-321. 

 Neelsen, F, (1883). 

 Ziehl, F. (1882). 



CARBOL FUCHSIN - HAEMATOXYLIN 

 For tubercle bacilli in mammalian tissue 



Technique: 



Tissues are fixed in Zenker or Flemming and embedded in 

 paraffin wax, L.V.N, or Celloidin. 



1. Paraffin sections are brought down to distilled water; then 

 stained one to five minutes in Solution A. 



2. Differentiate if necessary with acid alcohol, controlling under 

 the microscope, till nuclear detail is sharp and clearly defined; 

 wash thoroughly in water. 



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