STAINING, PRACTICAL AND THEORETICAL 



3. Immerse in 10% formalin, of a slightly acid reaction, for five 

 minutes. 



4. Immerse in the acid alcohol for five minutes. 



5. Flood the preparation with potassium permanganate and 

 allow the reagent to act until the sections turn brown (this usually 

 takes from two to five minutes). 



6. Immerse in the oxaHc acid for one minute. 



7. Stain with Ehrlich haematoxylin solution for two minutes; 

 then blue in tap water or in saturated lithium carbonate solution 

 aqueous. 



8. Stain in picro-acid fuchsin for two to five minutes; then 

 without washing: 



9. Dehydrate, clear and mount. 



Results: 



M. leprae: dark blue. Connective tissue fibres: red. Muscle: 

 yellow. Nuclei: brown. 



Reference: Fite, G. L. (i939)- 



CARBOL FUCHSIN - HAEMATOXYLIN 

 ORANGE G 



For demonstrating leprosy organisms together with neuro- 

 keratin of the myelin sheath 



Solutions required: 



A. Lugol's iodine. 



B. Carbol fuchsin (Ziehl Neelsen). 



C. Absolute alcohol 35 ^1. 



Distilled water 65 ml. 



Hydrochloric acid concentrated. . 0-5 ml. 



D. Ehrlich's haematoxylin. 



E. Strong ammonia solution (sp. gr. 



o-88o) I mi- 

 Distilled water 99 ml. 



F. Orange G aqueous 1%. 



160 



