STAINING, PRACTICAL AND THEORETICAL 



CARBOL FUCHSIN - METHYL GREEN 

 For demonstrating hyaline substance 



Solutions required: 



A. Carbol fuchsin (Ziehl Neelsen) . . 5 ml. 

 Distilled water . . . . • • 45 rnl* 



B. Methyl green, 1% in 5% aqueous 

 phenol 



Technique: 



1 . Material which has been fixed in any of the standard fixatives 

 is embedded in paraffin wax. 



2. Fix sections to slides : then bring down to distilled water as 

 usual. 



3. Stain in Solution A for fifteen to forty-five minutes. 



4. Wash with distilled water ; drain off^ excess ; then blot care- 

 fully. 



5. Dehydrate rapidly with absolute alcohol. 



6. Differentiate and counterstain in Solution B for two or three 

 minutes. 



7. Wash quickly with absolute alcohol. 



8. Clear in xylol and mount. 



Results: 



Hyaline substance is stained bright red while nuclei are light 

 green. 



Note: Carleton & Leach attributed the method to Russell. 



References : 



Carleton, H. M. & Leach, E. H. (1947), pp. 307-308. 

 Schmorl, C. G. (1928), p. 219. 



CARBOL THIONIN - PICRIC ACID 



(After Schmorl) 



For demonstrating bone canaliculi 



Solutions required: 



A. Decalcifying solution: 



Formalin 10% . . . . . . 100 ml. 



Nitric acid, cone. . . . . 15 ml. 



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