STAINING, PRACTICAL AND THEORETICAL 



the j3, y, and S toxins of CI. histolyticum, native collagen is attacked 

 by the /3 toxin only. 



{c) The author confirms the results of Evans and Prophet 

 (1950) in similar experiments on dentine with the enzymes of 

 CI. histolyticum. 



(d) In the original paper, to which reference should be made for 

 further information, it is suggested that possibly the method 

 could be extended by use of different coloured dyes structurally 

 related to chlorazol fast pink. In this connection I would suggest 

 the following dyes which are structurally related to chlorazol fast 

 pink: 



Chlorazol sky blue FF (Michrome No. 91) 



Chlorazol violet N ( „ ,,456) 



Chlorazol green ( ,, ,, 675) 



Another dye of the same group (disazo), which might be worth 

 trying is 



Chlorazol black E, Michrome No. 92 



Reference: Weatherell, J. A. (i960). 



CHRYSOIDIN 

 For the selective staining of mast cell granules 



Note- The author (Kiyoshi Harada) states that smears of abdominal fluid 

 mesentery and skin of mouse were fixed in methyl alcohol ethyl alcohol 20 /„ 

 formalin and 4% basic lead acetate solutions, dehydrated with alcohol and 

 embedded in paraffin wax. Also, cock's comb, umbilical cord of pig cartilage 

 matrix and chick embryo, goblet cells of mouse intestine and juxtaglomerular 

 granules of mouse kidney were used for the contrast materials. 



Method I 



Solution required: 

 Chrysoidin Y, 0-5% aqueous 



Technique : 



1. Remove paraffin wax from sections with xylol, and take 

 down to 80% alcohol in the usual way. 



2. Stain in the chrysoidin solution for five to ten minutes. 



170 



