SECTION TWO 



3. Rinse in distilled water. 



4. Dehydrate in 96% alcohol and two changes of absolute 

 alcohol. 



5. Clear in xylol, and mount in Canada balsam in xylol or in a 

 synthetic mountant such as D.P.X. or Cristalite. 



Results: 



Mast cell granules: deep brown to black in type I cell; brown 

 to yellow in type II cell. Nuclei and other tissue elements: 

 slightly yellowish. Glands in subcutaneous tissues: yellow. 



Note: The author states that in using simple aqueous solutions 

 of various basic dyes in investigating the staining of tissue con- 

 taining acid polysaccharides, it was found that only chrysoidin 

 stained the mast cell granules selectively. 



Method 2 

 Chrysoidin-eosin-light green-haematoxylin 



Solutions required: 



A. Alum haematoxylin (Carazzi) 

 Haematoxylin 

 Potash alum 

 Distilled water 

 Glycerine . . 

 Potassium iodate 



B. 



Light green SF, 1% aqueous 

 Distilled water 



o-i gm. 



5gm. 

 80 ml. 

 20 ml. 



0-02 gm. 



I ml. 

 49 ml. 



or. 



C. Eosin, 1% aqueous . . . . 0-5 ml. 

 Tap water . . . . . . 49-5 ml. 



Note: Solution C contains 1:10,000 of eosin, and 

 this may be further diluted, to as low as i :50,ooo, 

 with tap water, if desired. 



D. Chrysoidin Y, 0-5% aqueous 



Technique: 



1. Take sections down to 80% alcohol as usual. 



2. Stain in the alum haematoxylin for five to ten minutes. 



171 



