STAINING, PRACTICAL AND THEORETICAL 



3. Wash in tap water. 



4. Stain for three minutes in either solution B or C. 



5. Rinse in water. 



6. Stain in the chrysoidin solution for five to ten minutes. 



7. Rinse in water. 



8. Dehydrate with 96% alcohol and two changes of absolute 



alcohol; then clear and mount. 



Results: 



Mast cell granules: brown. Neuclei: greyish. Epidermis: 

 slightly yellow. Glands: yellow. Connective tissue: green or red. 



Method 3 

 Chrysoidin - periodic acid - Schiff 



Solutions required: 



A. Periodic acid, 0-7% aqueous* 



* See note {d), page 173 



B. Schiff 's reagent 



C. Sodium bisulphite, 0-52% aqueous 



D. (Optional) Alum haematoxylin (Carazzi) 



E. Chrysoidin Y, 0-5% aqueous 



Technique: 



1. Immerse in solution A for ten minutes. 



2. Rinse in water. 



3 Immerse in the Schiff 's reagent for fifteen to thirty minutes. 



4. Immerse for one minute in each of three changes of the 

 sodium bisulphite solution. 



5. Wash in running water for ten minutes. 



6. (Optional) Stain in the haematoxylin solution for five to 



ten minutes. 



7. Wash in water. 



8. Stain in the chrysoidin solution for ten minutes. 



9. Rinse in water. 



10. Dehydrate with 96% alcohol and two changes of absolute 



alcohol. , , , . 11 



11. Clear in xylol and mount in Canada balsam in xylol or in a 



neutral synthetic medium. 



172 



