STAINING, PRACTICAL AND THEORETICAL 



B. Lithium carbonate saturated aqueous 



C. Delafield or Ehrlich haematoxylin. 



Technique: 



1. Formalin or alcohol-fixed material may be embedded in 

 Celloidin or in paraffin wax, or frozen sections may be employed. 



2. Sections are mounted on slides and brought down to distilled 

 water as usual. 



3. Stain in the Congo Red solution for ten to thirty minutes. 



4. Immerse in the lithium carbonate solution for fifteen seconds. 



5. Decolorize in 80% alcohol until stain ceases to come away 

 in clouds. 



6. Wash in running water for fifteen minutes; then stain with 

 EhrUch or Delafield haematoxylin for five to ten minutes. 



7. Wash in tap water; dehydrate in the usual manner; clear 

 in xylol and mount. 



Note: If Celloidin sections are used dehydration should be 

 carried out with isopropyl alcohol in place of ethyl alcohol. 



Results: 



Amyloid, red; nuclei, blue. 



References : 



Bennhold, H. (1922). 

 Cowdry, E. V. (1952). 

 Hass, G. M. (1942)- 

 Taran, A. G. (1936/7)- 



CONGO RED - ANILINE BLUE - ORANGE G 



For elastic fibres 



Solutions required: 



A. Aluminium chloride, 2% aqueous. 



B. Congo Red ^ gm. 



Sodium citrate 2-5 gm. 



Glycerin ^ "^1- 



Distilled water 97 ml. 



174 



