SECTION TWO 



C. Aniline Blue, aqueous . . . . i'5 gm. 



Orange G . . . . . . 2-25 gm. 



Resorcinol . . . . -3 gi^- 



Phosphomolybdic acid i% aqueous 100 ml. 



Technique: 



Tissues should be fixed in 10% formalin, and frozen sections 

 should be employed. 



1. Wash sections in water; then immerse them in Solution A 

 for ten minutes. 



2. Wash with water and drain; then stain in the Congo Red 

 solution for ten minutes. 



3. Wash with tap water ; then plunge the slide into a dish of tap 

 water and agitate it there for ten seconds. 



4. Wash again with tap water ; then stain from five to ten min- 

 utes in the Aniline Blue-Orange G solution (Solution C above). 



5. Rinse carefully in tap water; drain well and blot. 



6. Dehydrate in absolute alcohol ; clear in origanum oil ; wash 

 in xylol and mount. 



Results: 



Elastic fibres: bright red. Fibrin: dark blue. Erythrocytes: 

 yellowish orange. 



Reference: Krajian, A. A. (1941). 



CONGO RED - EHRLICH HAEMATOXYLIN 

 For eleidin and keratohyalin 



Solutions required: 



A. Congo Red 0-05% aqueous. 



B. Ehrlich haematoxylin. 

 Techiique: 



1. Material should be fixed in absolute alcohol and embedded 

 in paraffin wax. 



2. Sections not more than 5/1 thick are mounted on slides and 

 brought down to distilled water as usual. 



3. Stain for five to ten minutes in the Congo Red solution. 



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