STAINING, PRACTICAL AND THEORETICAL 



4. Rinse in distilled water; then stain for five to ten minutes in 

 Ehrlich haematoxylin. 



5. Blue in tap water in the usual manner. 



6. Dehydrate ; clear in xylol and mount. 



Results: 



Eleidin is stained red, while nuclei and keratohyalin are blue. 



Reference: Carleton, H. M. & Leach, E. H. (i947). P- 287. 



CONGO RED 

 A simple stain for beta cells of the hypophysis 



Solutions required: 



A. Congo red i gm- 



Distilled water 100 gm. 



B. Lithium carbonate, saturated 



aqueous 



C. Haematoxylin (Harris) 



D. Absolute alcohol . . • • 7° "^1- 



Distilled water 30 ml. 



HCl, cone. . . . . . • I rnl- 



E. Tap water substitute (Scott), as 



recipe, page 546 



Technique: 



1. Fix material in 10% formaHn and embed in paraffin wax. 

 Note: After adequate fixation the time the material is left in 



formalin, within reasonable limits, does not affect the result. 



2. Cut sections at 6/x and attach them to slides. 



3. Remove paraffin wax with xylol. 



4. Wash in absolute alcohol. 



5. Wash in 90% alcohol. 



6. Wash in 70% alcohol. 



7. Wash in distilled water. 



176 



