STAINING, PRACTICAL AND THEORETICAL 



CRESOFUCHSIN - CARMINE - ANILINE BLUE - 



ORANGE G 



For demonstrating the various components of the 



Hypophysis 



Solutions required: 



A. Cresofuchsin, i% in absolute 



alcohol containing 2% of cone. 

 HCl 



B. Alum carmine (Mayer). 



C. Orange G 2 gm. 



Phosphomolybdic acid . . . . i gm. 

 Distilled water 100 ml. 



D. Phosphomolybdic acid 5% aqueous. 



E. Aniline Blue 0-2% aqueous. 



Technique: 



I. Fix in 10% formalin; harden and dehydrate in graded alco- 

 hols; clear in chloroform: embed in paraffin wax. 



2.' Sections are brought down to 70% alcohol and stained for 

 two to twenty-four hours in Solution A. 



3. Wash quickly with distilled water; then stain with Solution 

 B for three hours, afterwards washing with distilled water. 



4. Differentiate and stain the acidophil cells for five minutes 

 with Solution C; then rinse in distilled water. 



5. Immerse in Solution D for two minutes; then blot dry. 



6. Stain ten to twenty minutes with Solution E. 



7 Rinse in distilled water; differentiate with 75% alcohol until 

 no more stain comes out; then dehydrate; clear in xylol and 

 mount. 



Results: 



Chief cells: blue to grey. Pregnancy cells, blue with small bright 

 yellow granules; basophiles with coarse reddish blue granules. 

 Epithelium of the pars intermedia and pars tuberalis: variable. 

 Collagen fibre: intense blue. Glia fibres, blue-grey; axons, 

 occasionally black. 



Reference: Berblinger & Bergdorf (i935)- 



178 



