STAINING, PRACTICAL AND THEORETICAL 



CRESYL FAST VIOLET 

 A durable Nissl stain for frozen and paraffin sections 



(After R. C. Fernstrom, 1958) 



Solutions required: 



A. Albrecht's alcoholic gelatine 



(recipe page 527) 



B. Cresyl fast violet . . . . . , 5 gm. 

 Distilled water . . . . . . 100 ml. 



C. Acetic acid, 1% aqueous 



D. Solution B . . . . • • 5 nil' 

 Solution C . . . . • • 5 ^^ 

 Distilled water , . . . . . 90 ml. 



Note: This solution should be prepared as and 

 when required for immediate use. 



E. Celloidin 0-5% in equal volumes 



of absolute alcohol and ether 



F. Absolute alcohol . . • • 50 ml. 

 Chloroform . . . . • • 50 ml. 



Technique: 



{a) Frozen sections 



1. Frozen sections are cut at 25-50/x and stored in 10% 

 formalin. 



2. Rinse in distilled water. 



3. Immerse in Albrecht's alcoholic gelatine for at least five 

 minutes. 



4. Transfer the sections, by means of a fine camel hair brush 

 to slides. 



5. Drain off excess liquid by tilting the slide and carefully 

 blotting around the edges of the section. 



6. Leave the preparation exposed to the atmosphere until it is 

 almost dry. 



7. Gently blot with filter paper. 



8. Immerse at once in 95% alcohol. 



9. Dehydrate in absolute alcohol. 



10. Immerse in solution E (o'5% celloidin) for at least one 

 minute. 



180 



