SECTION TWO 



DAHLIA ACETIC 

 For mast cell granules in sections 



Dissolve by heating in a flask, lightly plugged with cotton-wool, 

 on a water bath. Allow to cool; then filter. 



Technique: 



1. Fix tissues in absolute alcohol and embed in Celloidin. 



2. Immerse sections in the staining solution for twelve hours. 



3. Differentiate in 95% alcohol. 



4. Clear in origanum oil and mount in balsam or in cristalite. 



Results: 



Granules of mast cells are stained reddish violet 



Reference: Romeis, B. (1948), p. 321. 



DIGITONIN REACTION 

 For cholesterol 



Reagents required: 



A. Digitonin, saturated in 70% alcohol 



Note: Digitonin is only slightly soluble. 



B. Paraffin wax, M.Pt. 52-56° C . . 95 gm 

 Glyceryl monostearate . . 



C. Bismuth trichloride 

 Acetyl chloride 

 Nitrobenzene, anhydrous 



D. Acetyl chloride 

 Nitrobenzene, anhydrous 



185 



5gm. 

 0-2 gm. 



I ml. 

 100 ml. 



10 ml. 

 90 ml. 



