SECTION TWO 



ELASTIN STAIN (Sheridan) 



This stain has an advantage over Weigert's elastin stain in that 

 the solution may be kept for reasonably long periods without 

 deterioration. 



The staining procedure is the same as for Weigert's elastin stain. 



Results: 



Elastic fibres are stained green to greenish black. 



Reference: Sheridan, W. F. (1929). 



ELASTIN - TRICHROME STAIN 



For the demonstration of elastic, smooth muscle and 

 collagenic fibres with equal clarity, particularly in the walls 



of blood vessels 



Solutions required: 



A. Weigert's elastin stain 



Weigert's elastin stain powder . . i gm. 

 Hydrochloric acid, cone, pure . . 2 ml. 

 Absolute alcohol . . . . 100 ml. 



Dissolve the stain by boiling for two minutes in a 

 flask, plugged lightly with cotton-wool, on a water 

 bath. Allow to cool; then filter; make the volume 

 up to 100 ml. with absolute alcohol; then add the 

 acid. Alternatively the solution may be prepared as 

 described on page 188. 



Note: This solution deteriorates after three or 

 four weeks. 



B. Ehrlich haematoxylin. 



C. Ponceau-acid fuchsin (Masson): 



Acid fuchsin . . . . . . 0-3 gm. 



Ponceau de xylidine . . . . 0-7 gm. 



Distilled water 100 ml. 



Glacial acetic acid . . . . i ml. 



D. Phosphotungstic acid 3% aqueous. 



E. Light Green 1% aqueous. 



G* 189 



