STAINING, PRACTICAL AND THEORETICAL 



Technique: 



1. Paraffin sections are mounted on slides and brought down to 

 distilled water in the usual manner; then immersed in Weigert's 

 elastin stain in a staining jar for one hour. 



2. Wash rapidly in acid alcohol; then dehydrate and differ- 

 entiate in absolute alcohol until the sections appear only famtly 

 red. 



3. Immerse in 70% alcohol, followed by distilled water. 



4. Stain in Ehrlich haematoxylin for eight to ten minutes ; then 

 differentiate in water for five minutes. 



5. Stain in Ponceau-acid fuchsin for five minutes. 



6. Washthoroughlyin3%phosphotungsticacid; then immerse 

 in the phosphotungstic acid for ten minutes. 



7. Wash thoroughly in distilled water; then stain with Light 

 Green for two to five minutes; then without washing: 



8. Flood the preparation with 1% acetic acid and allow it to act 

 for three minutes; pour off excess ; then without washmg : 



9. Dehydrate; clear; mount in D.P.X. 



Results: 



Elastic tissue stained blue-black; smooth muscle, red; collagen, 



green. 



Reference: Mendeloff, J. & Blechman, H. (i943)- 



EOSIN B - ANILINE BLUE (Rhodocyan technique) 

 A one-step staining method for anterior pituitary 



(After G. G. Glenner & R. D. Lillie, 1957) 



Solutions required: 



A. Eosin B, aqueous 10% 



B. Aniline blue, aqueous 10% 



C. Citric acid, M/io 



D. Disodium hydrogen phosphate, M/5 



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