STAINING, PRACTICAL AND THEORETICAL 



islets by adjusting the buffer to pH 3-6 and staining for only three 



minutes at 60° C. 



(c) The name "Rhodocyan " (rose-dark blue) was given to 

 the technique by the authors (Glenner & Lillie). 



(d) Using the modification mentioned above (note b) the 



results are: ^ , , n j 



Duodenal enterchromaffin cells: blue-black. Goblet cells and 

 mucin of gastric mucous cells: light blue. Paneth cell granules: 

 blue-black. Alpha cells of pancreatic islets: red. Beta cells: 

 purple. Collagen and reticulum: deep blue. Striated and smooth 

 muscle: purple-violet. Neurokeratin: pale pink. Endoneurium: 

 blue Axis cylinder: grey. Eosinophil granules: red. Internal 

 root sheath of hair follicle: orange-red. External root sheath: 



violet. • T, A 



(e) In the original paper 1% stock solutions of eosin B and 

 aniline blue are stipulated, but since a 1% aqueous solution of 

 either stain is liable to attack by air-borne micro-organisms, 

 which bring about their decomposition in a relatively short time 

 I suggest dilutions be made as and when required from 10% 

 solutions, which are immune to attack by moulds and other 

 micro-organisms and remain stable for several years. 



(/) For more detailed information regarding the Rhodocyan 

 technique readers should consult the original paper which carries 

 three photomicrographs. 



References : 

 Glenner, G. G. & Lillie, R. D. (i9S7)- 

 Lillie, R. D. (i9S4)P-4i. 



EOSIN -LIGHT GREEN - CHROMIC ACID 

 For demonstrating tryptophane-containing proteins 



Solutions required: 



A. Chromium trioxide, 4% aqueous 



B. Phosphomolybdic acid, 1% aqueous 



C. Eosin, yellowish, 1% aqueous 



D. Light green SF, yellowish, 1% aqueous 



Technique: 



1. Immerse sections in solution A for thirty minutes. 



2. Wash in running water for twenty to thirty minutes. 



192 



