STAINING, PRACTICAL AND THEORETICAL 



(c) The original author states that the combination azur A-erie 

 garnet produces colour reactions closely simulating haematoxylin 

 and eosin. 



(d) Readers are referred to the original paper for more detailed 

 information. 



Reference: Geschickter, C. A. (1930). 



ERYTHROSIN - WATER BLUE - HAEMATOXYLIN 



For vaginal smears to facilitate analysis of the menstrual 



cycle 



Solutions required: 



A. Haematoxylin (Ehrlich) 



B. Erythrosin, 0-5% aqueous 



C. Phosphomolybdic acid, 2% 



D. Water blue, 2% aqueous 



Technique: 



1. Fix wet smears in a mixture consisting of equal volumes of 

 absolute alcohol and ether for five to fifteen minutes. 



2. Rinse successively in 90%, 70%, and 50% alcohols. 



3. Rinse in distilled water. 



4. Stain for twenty minutes in Ehrlich's haematoxylin. 



5. Rinse in distilled water. 



6. Differentiate in 0-5% hydrochloric acid. 



7. Wash in running tap water for a few seconds. 



8. Blue in saturated aqueous lithium carbonate. 



9. Wash well in tap water. 



10. Immerse in the erythrosin solution for fifteen minutes. 



11. Wash with tap water. 



12. Mordant in the phosphomolybdic acid for five minutes. 



13. Wash well with tap water; then rinse with distilled water. 



14. Stain with water blue (solution D) for about twenty 

 minutes. 



15. Wash well with tap water. 



16. Dry thoroughly in the air and mount in D.P.X. or other 

 neutral synthetic mountant. 



196 



