SECTION TWO 



Results: 



Nuclei: dark blue. Cytoplasm: red, blue or pale purple, 

 depending upon the condition of the cell. The proportion of the 

 cells with either red or blue cytoplasm was found to depend on 

 the stage of the menstrual cycle. Pale purplish cytoplasm was 

 found in smears made half an hour before menstrual bleeding 

 started. 



The author (C. Van Duijn, jnr.) claims that the method facil- 

 itates cycle diagnosis. 



Reference: Van Duijn, jnr., C. (1956). 



ETHYL VIOLET - BIEBRICH SCARLET 

 For pepsinogen granules in gastric mucosa 



Solutions required: 



A. Stock solution. 



Ethyl violet-Biebrich scarlet 1% 

 in 20% alcohol. 



B. Staining solution. 



Stock solution (as above) . . 0-5 ml. 

 Alcohol 20% 100 ml. 



Technique: 



1. Tissues should be fixed in Regaud's fluid, washed in running 

 water, dehydrated, cleared and embedded in paraffin wax in the 

 usual manner. 



2. Fix sections to slides, dewax and pass through descending 

 grades of alcohol down to distilled water. 



3. Stain for twenty-four hours in Solution B in a covered 

 staining jar. 



4. Drain and wipe off excess liquid. 



5. Differentiate for about half an hour with a mixture con- 

 sisting of equal volumes of clove oil and xylol, controlling by 

 examination under the microscope at intervals. 



6. Wash well with several changes of xylol ; mount in cristalite. 



Results: 



Pepsinogen of the pepsin-forming cells is stained violet, while 

 parietal cells are red. 



Reference: Bowie, D. J. (1935/6). 



197 



