SECTION TWO 



3. FALGOG METHOD 



For demonstrating erythrocytes, minute blood vessels and other 

 strongly erythrophile elements of animal and plant tissues. 



Solutions required: 



*A. Acid fuchsin 0-5% (Michrome No. 5) in 

 4% aqueous acetic acid 

 (See note [a), page 199.) 



B. Light green (Michrome No. 240), 0-125% 



aqueous 



C. Acetic acid, 2% aqueous 



D. Orange G (Michrome No. 411), 0-5% 



aqueous 



Techtiique: 



1. Stain sections or smears in solution A for five minutes. 



2. Wash in running water for five minutes. 



3. Stain in the light green (solution B) for five minutes. 



4. Wash in running water for five minutes. 



5. Immerse in the dilute acetic acid (solution C) for five 

 minutes. 



6. Wash in running water for five minutes. 



7. Immerse in the orange G (solution D) for five minutes. 



8. Wash in running water for five minutes. 



9. Dehydrate through the usual graded alcohols. 



10. Clear in xylol. 



11. Mount in D.P.X. or Emexel or Canada balsam in xylol. 



Results: 



The orange G obliterates the Falg colours but leaves the 

 strongly erythrophile (MacConaill, 1949, 195 1) elements stained 

 red according to their degree of erythrophilia. The red blood 

 corpuscles stand out very clearly, facilitating the tracing of 

 minute blood vessels throughout their course. 



Notes: 



(a) Without the protective acid bath (step 5) all the colours 

 are obliterated by the orange G. 



201 



