SECTION TWO 



Results: 



Similar to those given by the Falgog method: see " Results " 

 and " Notes ", page 201-202. 



Notes: 



Readers should refer to pages 76-90 of this book, and to the 

 authors' original papers listed in the bibliography, for further 

 information. 



References : 



MacConaill, M. A. & Gurr, E. (1960a). 

 Gurr, E. & MacConaill, M. A. (19606). 



FAVIOLIC ACID REACTIONS 



I. BASIC FAVIOL METHOD 



A simple universal polychrome staining method applicable to 

 animal and plant tissues. 



A. Acid fuchsin (Michrome No. 5) 0-5 gm. 

 Acetic acid, 4% aqueous . . 100 ml. 



(See note, page 199.) 



B. Violamine 3B (Michrome No. 



161) 0-5 gm. 



Water . . . . . . . . 100 ml. 



Techfiique: 



1 . Stain sections or smears in solution A for five minutes. 



2. Wash in running water for five minutes. 



3. Stain in solution B (violamine) for five minutes. 



4. Wash in running water for five minutes. 



5. Dehydrate through the usual graded alcohols. 



6. Clear in xylol. 



7. Mount in D.P.X. or Emexel or Canada balsam in xylol. 



Results: 



Similar to those produced by the Falg technique but the 

 violet and blue colours are deeper than the corresponding shades 

 obtained by using the Falg method. 



203 



