STAINING, PRACTICAL AND THEORETICAL 



Results: 

 Argentaffine granules : black. 



References : 



Jacobson, W. (1939). 

 Fontana (19 12). 



» 



FONTANA STAIN - SILVER NITRATE 

 For reticular and collagen fibres 



Solutions required: 



A. Strong ammonia solution (sp. gr. 



o-88o) . . . . . . . . 10 ml. 



Distilled water . . . . . . 90 ml, 



B. Potassium permanganate 0-5% aqueous. 



C. Oxalic acid 1-5%. 



D. Silver nitrate 5% aqueous. 



E. Fontana stain (silver diaminohydroxide, as page 415). 



F. Aniline oil.. .. .. .. i volume 



Xylol . . . . . . . . I volume 



Technique: 



1. Frozen sections not thicker than 10^ are fixed in 10% for- 

 malin and afterwards washed in three changes of water for fifteen 

 minutes in each. 



2. Immerse in Solution A at 60° C. for fifteen minutes, in an 

 oven. 



3 . Rinse well in three changes of distilled water. 



4. Immerse in the potassium permanganate for three or four 

 minutes. 



5. Rinse with distilled water for about ten or twenty seconds. 



6. Decolorize with the oxalic acid solution until the brown 

 colour just disappears; then wash well in distilled water. 



7. Immerse in silver nitrate solution in the dark for an hour. 



8. Wash well with two changes of distilled water in the dark. 



9. Immerse in Fontana's stain for fifteen minutes at 60° C. in 

 the dark. 



208 



