SECTION TWO 



10. Wash rapidly in three changes of distilled water. 



11. Immerse in 30% formalin for two or three minutes at 

 60° C. 



12. Wash thoroughly in running tap water; then transfer to 

 slides. 



13. Blot away excess water. 



14. Dehydrate with two changes of absolute alcohol. 



15. Clear in the aniline-xylol (Solution F, above), 



16. Wash with xylol; mount in Clearmount. 



Results. 



Reticulum is stained black, while collagen is brown. 



N.B. : Sections must be handled with glass needles throughout 

 this technique, as contact with metal instruments will ruin the 

 preparations. 



Reference: Krajian, A. A. (1933). 



FUCHSIN ACID, ACETIC 



A simple and selective stain for both alpha and beta cell 

 granules of the anterior part of the pituitary 



Solutions required: 

 Acid fuchsin, 1% aqueous . . 100 ml. 



Acetic acid, glacial . . . . i ml. 



Technique: 



1. Fix material in Helly's fluid for sLx to twenty-four hours, 

 and embed in paraffin wax as usual. 



2. Fix sections to slides; dewax and carry down through the 

 alcohols to distilled water as usual. 



3. Immerse in the acetic acid fuchsin solution for one hour. 



4. Wash in running water. 



5. Examine under the microscope while the preparation is 

 still wet; if the beta cells are not bluish violet, return to the acetic 

 acid fuchsin solution for a further period of one hour, then wash 

 in running water and re-examine under the microscope. 



209 



