STAINING, PRACTICAL AND THEORETICAL 



Note: A staining time of from one to as much as twelve hours 

 is stipulated in the original paper, and it may, therefore, in some 

 cases, be necessary to repeat steps 3, 4, and 5 above, before the 

 desired results are obtained. 



6. Dehydrate through the usual graded alcohols. 



7. Clear in toluol and mount in Canada balsam, Cristalite, 

 Clearmount or D.P.X. 



Results: 



Alpha cell granules: red. Beta cell granules: blue-violet. 

 Reference: Petrovitch, A. (1953). 



FUCHSIN ACID -LIGHT GREEN 

 A rapid method for staining mitochondria 



Solu tiotis reqii ired : 



A. Hydrochloric acid N/i 



B. Fuchsin acid, 1% aqueous 



C. Light green SF, 1% aqueous 



Technique: 



1. Paraffin sections of formalin-fixed material are mounted on 

 slides and dewaxed with xylol as usual. 



2. Pass the slides through the usual graded alcohols into water. 



3. Hydrolyse for three minutes in the hydrochloric acid 

 (solution A), at 60° C. 



4. Rinse in water. 



5. Stain in the fuchsin acid solution for thirty seconds. 



6. Rinse in water. 



7. Counterstain in the light green solution for one to three 

 minutes. 



8. Rinse in water. 



9. Dehydrate with 95% and absolute alcohol. 



10. Clear in xylol. 



11. Mount in D.P.X. or in Canada balsam in xylol. 



210 



