SECTION TWO 



Results: 



The mitochondria stand out sharply stained purphsh red with 

 green peripheral wall. Chromatin and collagen are green. Muscle 

 tissue: purplish. Erythrocytes: brilliant red. 



Note: 



It may be of interest to compare the results obtained with 

 this technique with those obtained using the same dyes, but 

 under simpler conditions and without hydrolysis, in the techniques 

 of MacConaill & Gurr, pages 198-200. 



Reference: Novelli, A. (1959). 



FUCHSIN ACID (Altmann) - METHYL GREEN 

 For mitochondria in leucocytes of fixed blood smears 



Solutions required: 



A. Potassium dichromate . . . . 15 gm. 

 Distilled water . . . . . . 100 ml. 



B. Iodine 0-5% in 70% alcohol 



C. Sodium thiosulphate 0-5% aqueous 



D. Altmann's acid fuchsin 



E. Methyl green, 1% aqueous 



Technique: 



1. Make smears of fresh, unoxalated blood on scrupulously 

 clean slides, and fix by placing them at once in Helly's fluid and 

 leaving therein for twenty-four hours. 



2. Post-chrome in solution A for forty-eight hours. 



3. Immerse in solution B for two minutes. 



4. Rinse in 70% alcohol. 



5. Immerse in the sodium thiosulphate solution for one 

 minute. 



6. Wash in running water for several hours or overnight. 



7. Transfer to distilled water. 



8. Take a slide from the distilled water and blot or wipe away 

 the water from all parts of the slide except that covered by the 

 smear. 



9. Place the slide over the corner of a tripod, flood with solution 

 D and heat the preparation over a flame until fumes appear. 



211 



