SECTION TWO 



Technique: 



1. Small pieces of tissue, not more than 2 mm. in diameter, are 

 fixed for twenty-four hours in Altmann's Fluid. 



2. Wash for an hour in running water; then dehydrate; clear, 

 and embed in paraffin wax in the usual manner. 



3. Sections, not thicker than 4/^, are brought down to distilled 

 water; then stained for six minutes in Solution B. 



4. Pour off excess stain; then blot section carefully; then 

 differentiate and counterstain by flooding the preparation with 

 Solution C. 



5. Rinse quickly in 95% alcohol; then dehydrate with absolute 

 alcohol ; clear in xylol, and mount. 



Results: 



Mitochondria are stained crimson against a vivid yellow back- 

 ground. 



Reference: Altmann, R. (1904). 



FUCHSIN ACID - TOLUIDINE BLUE - AURANTIA 



For mitochondria 



H 



Solutions required: 



A. Champy's Fluid: 



Potassium dichromate 3% 

 Chromic acid 1% . . 

 Osmic acid 2% . . 



B. Pyroligneous acid 

 Chromic acid 1% 



C. Potassium dichromate 3%. 



D. Acid fuchsin 

 Aniline water 



E. Toluidine blue 0-5% aqueous. 



F. Aurantia 0-5% in 70% alcohol. 



213 



