STAINING, PRACTICAL AND THEORETICAL 



Technique: 



1. Tissues are fixed in Champy's Fluid for twenty-four hours; 

 then washed in running water for at least an hour. 



2. Immerse in Solution B for twelve to twenty hours ; then wash 

 in distilled water for thirty minutes. 



3. Mordant in Solution C for three days; then wash in running 

 water for twenty-four hours. 



4. Dehydrate; clear; embed in paraffin wax. 



5. Sections, 3 to 5^ in thickness are taken down to water in the 

 usual manner; then stained for six minutes by flooding the slide 

 with Solution D, and heating gently till vapour rises. 



6. Rinse in distilled water; then counterstain in Solution E. 



7. Rinse with distilled water; then stain with Solution F for 

 thirty to fifty seconds. 



8. Differentiate with 95% alcohol; dehydrate; clear in xylol 

 and mount. 



Results: 



Mitochondria are stained red; nuclei, blue. Background, 

 yellow. 



Reference: Kull, H. (1913)- 



GALLOCYANIN - ORCEIN - ACID ALIZARIN BLUE - 



ALIZARIN VIRIDINE 



A general stain for animal tissues 



Solutions required: 



A. Gallocyanin o-i gm. 



Chrome alum 5% aqueous . . 100 ml. 



Boil for ten minutes. Allow to cool; then make up 

 the volume to 100 ml., filter and add five or six drops 

 of formalin. 



B. Orcein 0-5% in 70% alcohol . . 99 ml. 

 Hydrochloric acid cone. . . i ml. 



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