SECTION TWO 



Results: 



As Giemsa stain but with more intense colouring. 



References : 

 Giemsa, G. (1904). 

 May & Griinvvald (1902). 

 Pappenheim, A. (1912, 1917). 



GOLD CHLORIDE - SUBLIMATE 



(After Cajal) 



For neuroglia fibres; for astrocytes in central nervous 



system 



Solutions required: 



A. Neutral formalin . . . . . . 15 ml. 



Ammonium bromide . , . . 2 gm. 



Distilled water . . . . • • 85 ml. 



B. Gold chloride (brown or yellow) 1% aqueous. 



C. Mercuric chloride 5% aqueous. 



D. Sodium thiosulphate 10% aqueous. 



Technique : 



1 . Fresh pieces of tissue are fixed for two to twenty-one days in 

 Solution A. 



2. Frozen sections are cut 15 to 30// thick. 



3. Rinse in several changes of distilled water. 



4. Immerse sections, flattened out and not lying on top of one 

 another, for three to four hours in a freshly prepared mixture con- 

 sisting of: 



Solution B . . . . • • 5 nil. 



Solution C . . . . • • 5 nil. 



Distilled water . . . . • • 30 ml. 



{Note: 3 ml. of the mixture is required for each 

 section) 



until the astrocytes are stained dark against a relatively light 

 background; the reaction should be controlled by microscopic 

 examination of a section while still wet. 



H* 221 



