STAINING, PRACTICAL AND THEORETICAL 



GREENSTEIN'S FIVE-DYE STAIN 



(After Greenstein, 1961) 

 A general purpose stain for sections and smears 



Solutions required: 



Dissolve the haematoxylin in the alcohol. Measure out the 

 glycerin and pour it into a 250 ml. bottle. Rinse the measuring 

 cylinder out with the haematoxylin solution, to remove adhering 

 glycerin. Add the haematoxylin solution to the glycerin in the 

 stock bottle. Dissolve the iron alum and the ferrous sulphate in 

 the water, by shaking: do not heat. Pour this solution into the 

 stock bottle. Shake well. 



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