SECTION TWO 



Technique: 



1. Carry sections through to distilled water as usual, removing 

 mercuric precipitate if a mercury-containing fixative has been 

 used. 



2. Immerse in solution G for three to five minutes, agitating 

 occasionally. 



3. Wash thoroughly in tap water. 



4. Immerse in solution H for two to three minutes, with 

 agitation. 



5. Rinse briefly in distilled water until the water runs clean. 



6. Treat with two changes of 95% alcohol. 



7. Dehydrate in absolute alcohol. 



8. Clear in xylol. 



9. Mount in a neutral synthetic mountant such as DPX, 

 or Clearmount, etc. 



Results: 



Nuclei: dark greyish purple to greyish brown. 



Cytoplasm: light greyish yellow to greyish brown. 



Collagen: bright green. 



Erythrocytes : orange-yellow. 



Muscle cytoplasm: greyish green to olive brown. 



Mucus: pale green to turquoise. 



Reference: Greenstein, J. S. (1961). 



Notes: 



{a) The author (Greenstein) states that another method added 

 to the many earlier variants of Mallory, Masson and Heidenhain 

 connective tissue stains appears to be justified if it is simpler, 

 more dependable or more versatile and can be successful even in 

 the hands of inexperienced technicians. It is claimed that this 

 method, which employs only two staining solutions and fewer 

 steps than many routine haematoxylin-eosin methods, gives results 

 comparable with those obtained by skilled workers with more 

 exacting methods. 



{b) It is stated that the method can be applied to vaginal and 

 cervical smears fixed in ether-alcohol (i: i), or isopropyl alcohol 

 for two minutes, 



(c) For further information the original paper should be con- 

 sulted. 



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