STAINING, PRACTICAL AND THEORETICAL 



9. Wash in distilled water. 



10. Immerse in the borax-ferricyanide solution for eighteen 

 hours at 37° C. 



11. Wash in water. 



12. Mount in Aquamount or glycerine jelly, sealing the edges 

 of the coverslip with two coats of Laktoseal. 



Results: 



Phospholipids and nucleoproteins are stained dark blue to 

 almost black. Mucin: dark blue. Fibrinogen: pale blue. Cyto- 

 plasm : pale yellow. 



Note: 



Substances stained dark blue or black by this technique, and 

 which take the stain just the same after the pyridine extraction 

 test (page 431) are not phospholipids. Substances that stain dark 

 blue to black with the acid haematein, but which are removed 

 by pyridine extraction, are phospholipids. Kanwar (1961), 

 however, observes that this is not necessarily so in all cases. 



Reference: Baker, J. R. (1946). 



HAEMATOXYLIN CHROME ALUM 



(After Gomori) 

 For lipofuscins 



Solutions required: 



A. Sulphuric acid, 5% aqueous 



B. Potassium permanganate, 1% 



aqueous 



C. Solution A . . . . . . 3 ml. 



Solution B . . . , . . 47-5 ml. 

 Water 49-5 ml. 



D. Oxalic acid, 1% aqueous 



E. Haematoxylin, 10% in absolute 



alcohol 



228 



