SECTION TWO 



Results: 



Connective tissue: green. Striated muscle: brick red. Smooth 

 muscle: reddish violet. Nerves: blue-grey. Ganglion cells: violet. 

 Erythrocytes: orange. Cardiac conductive tissue is easily dis- 

 tinguishable from cardiac muscle as it takes a lighter shade of 

 staining. 



Note: Azophloxine is used here as a substitute for ponceau de 

 xylidine in Goldner's modification of Masson's technique. 



The stain is also suggested in place of Eosin as a counterstain 

 for use with haematoxylin. The advantages of using azophloxine 

 are that it gives clear and delicate pictures and it does not overstain, 

 if the recommended procedure is followed. When azophloxine 

 is to be used merely as a counterstain for haematoxylin the pro- 

 cedure is as follows : 



I. Proceed as steps, i, 2, 3, 4, and 5 (above). 

 II. Rinse in distilled water. 



III. Dehydrate with three changes of absolute alcohol; then 

 clear and mount. 



Reference: Halpern, M. H. (1954). 



HAEMATOXYLIN - BASIC FUCHSIN 



For haemofuscin, melanin and haemosiderin in animal 



tissues 



Solutions required: 



A. Haematoxylin (Ehrhch). 



B. Basic fuchsin 0-5% in 50% alcohol. 



Technique: 



Tissues may be fixed in Zenker or in absolute alcohol or in 10% 

 formalin. Paraffin or Celloidin sections may be employed. If 

 Zenker's fixative is used it will be necessary to remove mercury 

 deposits in the usual manner. 



1 . Stain for five to ten minutes in Ehrlich haematoxylin. 



2. Wash well in tap water, then several times in distilled water. 



3. Stain from five to twenty minutes in the basic fuchsin solu- 

 tion; then pour off excess stain and wash well in distilled water. 



4. Differentiate in 95% alcohol; then dehydrate in absolute 

 alcohol; clear in xylol and mount in balsam. 



231 



