SECTION TWO 



HAEMATOXYLIN - EOSIN 

 For general staining 



Solutions required: 



A. Haematoxylin (Delafield or 



Ehrlich) 



B. Eosin, yellowish, i%, aqueous. 



Technique: 



1. Tissues should be fixed in Zenker, Bouin or io% formalin 

 and embedded in paraffin wax. 



2. Sections are brought down to distilled water; then stained 

 with Delafield haematoxylin for ten minutes. 



3. Wash and immerse in tap water for about five minutes until 

 the section appears blue to the naked eye. 



4. Wash rapidly with distilled water ; then stain for one to two 

 minutes with 1% eosin yellowish, aqueous. 



5. Wash quickly with distilled water; then dehydrate with 

 95% and absolute alcohol. 



6. Clear in xylol and mount. 



Results: 



Nuclei are stained blue; cytoplasm, pink. 



Notes: 



Lee (1913) states that Delafield haematoxylin is frequently 

 attributed erroneously to Grenacher or Pruden. Conn & Darrow 

 (1947) state that the Delafield haematoxylin-eosin method was 

 introduced by Pruden (1885) in a note without a title. 



References : 



Delafield, F. (1885). 

 Ehrlich, P. (1886). 



HAEMATOXYLIN (Ehrlich) 

 For keratohyalin 



Solutions required: 



A. Haematoxylin (Ehrlich). 



B. Potass, permanganate 0'i%. 



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