STAINING, PRACTICAL AND THEORETICAL 



Technique: 



1. Material should be fixed in io% formalin and embedded in 

 paraffin wax. 



2. Fix sections to slides and bring down to distilled water as 

 usual. 



3. Stain in Ehrlich haematoxylin for ten minutes. 



4. Pour off excess stain ; then rinse and blue in tap water. 



5. Immerse in potassium permanganate o-i% for ten seconds; 

 then wash well with water. 



6. Dehydrate; clear; mount in balsam. 



Results: 



Keratohyalin is stained blue-black while the other elements are 

 unstained or faintly stained. 



Reference: Cowdry, E. V. (1952). 



HAEMATOXYLIN - GENTIAN VIOLET - IODINE 



(Gram-Weigert method) 



For demonstrating Gram-positive bacteria 

 and fibrin in sections 



Solutions required: 



A. Haematoxylin (Delafield). 



B. Aniline gentian violet 



C. Lugol's iodine. 



D. Aniline oil 20 ml. 



Xylol 10 ml. 



E. Erythrosin 5% in absolute alcohol. 



Technique : 



1. Tissues may be fixed in 10% formalin or in Zenker, if the 

 latter is used then mercuric precipitates must be removed from 

 the sections by the standard technique. 



2. Fix sections to slides; dewax with xylol and pass through 

 descending grades of alcohol to water in usual manner. 



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