SECTION TWO 



3. Stain in the haematoxylin solution for five to twenty minutes. 



4. Rinse quickly in acid alcohol. 



5. Immerse in a large volume of tap water for two to five min- 

 utes. 



6. Stain in the aniline gentian violet for two to five minutes. 



7. Pour off excess stain, and without washing, blot the slide 

 carefully. 



8. Flood with Lugol's iodine and allow the solution to act for 

 two to five minutes, 



9. Pour off excess, and without washing, blot dry carefully. 



10. Decolorize for a few seconds with Solution D. 



11. Flood the preparation with erythrosin (Solution E, above) 

 and allow the stain to act from one half to one minute. 



12. Pour off excess stain and wash the preparation with Solution 

 D. 



13. Rinse well with xylol; drain off excess and blot dry care- 

 fuUy. 



14. Mount in balsam or CristaHte or Clearmount. 



Results: 



Nuclei: blue. Fibrin and Gram-positive organisms: purplish 

 blue. 



References : 



Weigert, C. (1887). 



Mallory, F. B. (1938), p. 272. 



HAEMATOXYLIN (Heidenhain) - EOSIN 

 A general tissue stain 



Solutions required: 



A. Iron alum . . . . • • 3 gm- 

 Distilled water . . . . . . 100 ml. 



Dissolve by shaking. 



B. Haematoxylin 10% in absolute 



alcohol (which has been ripened 



for three months or longer) . . 5 ml. 



Distilled water . . . . • • 95 ml* 



C. Eosin yellowish 1% aqueous. 



235 



