STAINING, PRACTICAL AND THEORETICAL 



Technique: 

 Fix in Zenker. Embed in paraffin wax. 



1. Sections are brought down to distilled water, then mor- 

 danted in Solution A for one half to three hours. 



2. Wash in tap water; stain one to three hours in Solution B; 

 then rinse in tap water. 



3. Differentiate in Solution A, controlling by examination under 

 the microscope. 



4. Wash in running water for five to ten minutes. 



5. Stain with Solution C for one to three minutes. 



6. Wash in tap water; dehydrate; then clear in xylol and 

 mount. 



Results: 



Nuclei are stained black; cytoplasmic structures, pink. 



Reference: Heidenhain, M. (1892). 



HAEMATOXYLIN 

 For the identification of lipines 



Solutions required: 



A. Potassium bichromate 5% aqueous. 



B. Haematoxylin (Kultschitzky) 



C. Weigert's borax ferricyanide 



Potassium ferricyanide . . . . 2-5 gm. 



Borax . . . . . . . . 2 gm. 



Distilled water . . . . . . 100 ml. 



Technique. 



1. Tissues are fixed for twelve to twenty-four hours in 10% 

 formalin in normal saline. 



2. Wash for several hours in running water. 



3. Make frozen sections and collect them in distilled water. 



4. Immerse in Solution A for twenty-four to forty-eight hours 

 at 37° C. 



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