SECTION TWO 



5. Wash in several changes of distilled water, handling the sec- 

 tions with care (as they become brittle after immersion in Solution 

 A). 



6. Immerse in Solution B for four to six hours at 37° C. 



7. Wash in distilled water. 



8. Differentiate in Solution C, controlling under the micro- 

 scope, until the ground cytoplasm is changed from black to yellow. 

 This process takes several hours. 



9. Wash thoroughly in five or six changes of distilled water; 

 then mount in glycerine jelly or Aquamount. 



Result : 



Lecithin and other lipines are stained black to deep blue (light 

 blue coloration should not be taken as positive). Lipids and 

 other tissue constituents are colourless. 



Reference: Dietrich, A. (1910). 



HAEMATOXYLIN 



(After Kultschitzky) 



(Weigert's modification) 



For finer studies of cortical architecture and for total 



brain sections 



Solutions required: 



A. Weigert's Secondary Mordant: 



Cupric acetate neutral, normal . . 5 gm. 

 Fluorchrome . . . . . . 2-5 gm. 



Distilled water . . . . . . 100 ml. 



Boil ; allow to cool ; then add : 



Glacial acetic acid . . • • 5 ml- 



B. Haematoxylin (Kultschitzky). 



C. Lithium carbonate, saturated, 



aqueous . . . . . . 100 ml. 



Potassium ferricyanide 1% aqueous 10 ml. 



237 



