SECTION TWO 



HAEMATOXYLIN - PICRO PONCEAU S 



A selective stain for collagen and connective tissue in place 

 of Haematoxylin - Van Gieson 



Solutions required: 



A. Haematoxylin (Heidenhain or Ehrlich). 



B. Picro Ponceau S {Curtis): 



Ponceau S i% aqueous . . . . lo ml. 



Picric acid i% aqueous . . . . 86 ml. 



Acetic acid i% aqueous . . • • 4 ml- 



Technique: 



Proceed exactly as for Haematoxylin (Ehrlich) - Van Gieson or 

 Haematoxylin (Heidenhain) - Van Gieson (pages 243-4). 



Results: 



Identical with Haematoxylin - Van Gieson. 



Note: It is claimed that unlike Van Gieson, Picro Ponceau 

 does not fade when mounted in Canada balsam. 



References : 



Carleton, H. M. & Leach, E. H. (1947). 

 Curtis, F. (1905). 

 Leach, E. H. (1946). 



HAEMATOXYLIN (Weigert) SCARLET R 



For demonstrating fatty acids crystals, soaps and neutral 



fats in fat necrosis 



Solutions required: 



A. Formalin io% saturated with calcium salicylate. 



B. Copper acetate io% aqueous. 



C. Weigert haematoxylin, A. 



D. Weigert haematoxylin, B. 



E. Borax 0-2% aqueous . . . . i litre 

 Potassium ferricyanide . . . . 2-5 gm. 



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