SECTION TWO 



HICKSON'S PURPLE - VICTORIA GREEN 

 A general stain, particularly suitable for class work 



Solutions required: 



A. Hickson's Purple saturated aqueous. 



B. Victoria green, G. saturated in 70% alcohol. 



Technique : 



1. Fix sections to slides; dewax and take through the alcohols 

 down to distilled water as usual. 



2. Stain for ten minutes in the Hickson's purple. 



3. Rinse in distilled water. 



4. Stain in the victoria green for half to one hour. 



5. Rinse in 70%, followed by 90% alcohol. 



6. Clear in xylol and mount. 



Results: 



Nuclei are sharply defined, purple. Erythrocytes sharply 

 defined, stained vivid green, against a|general blue-purple back- 

 ground. 



Note: This method is an improvement on Hickson purple used 

 alone. 



Reference: Cannon, H. G. (1941). 



HITCHCOCK AND EHRICH'S MIXTURE 

 For plasma cells, etc. 



Technique: 



1. Fix in Zenker-acetic acid, corrosive sublimate, but not in 

 Miiller or formahn. 



2. Paraffin sections are brought down to 90% alcohol; then 

 passed through a solution of iodine in 90% alcohol. 



3. The iodine is removed by passing through the graded 

 alcohols to water and finally washing for fifteen minutes in running 

 water. 



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