STAINING, PRACTICAL AND THEORETICAL 



5. Seal the edges of the coversHps with soft paraffin wax (M.P. 

 38° C), and examine under the microscope. 



Results: 



Basophilic granules: brilliant scarlet. Eosinophilic granules: 

 yellow to light orange. Neutrophilic granules: salmon colour. 

 Mitochondria: small blue dots or rods. Nuclei: unstained. 



Note: The proportion of the two stains may be varied to suit 

 the particular specimen ; for instance, specimens very rich in cells, 

 such as leucaemic blood, need more concentrated mixtures of the 

 stains. 



Reference: Lightwood, Hawksley & Bailey (1935). 



JENNER STAIN 

 For blood-forming organs 



Solutions required: 



A. Formol- Saline. 



Formalin, cone. (i.e. 40% for- 

 maldehyde) . . . . . . 100 ml. 



Sodium chloride, A.R 8-5 gm. 



Distilled water i litre 



Acid sodium phosphate, mono- 

 hydrate, A.R 4 gm. 



Anhydrous disodium phosphate, 



A.R 6-5 gm. 



B. Jenner stain. 



Technique: 



1 . Fix pieces of tissue for two or three days in Solution A. 



2. Dehydrate in ascending grades of alcohol as usual; clear; 

 embed in paraffin wax. 



3. Fix sections, not exceeding 5^ in thickness, to slides; dewax; 

 pass through the usual descending grades of alcohol to distilled 

 water which has been buffered to pH 7-0. 



4. Stain for forty-five minutes in a grooved, stoppered staining 

 jar, with a mixture consisting of equal volumes of Jenner stain 

 and distilled water, buffered to pH 7.0. 



250 



