SECTION TWO 



5. Differentiate and dehydrate with absolute alcohol. 



6. Clear in xylol and mount in Cristalite or D.P.X. 



Results: 



Neutrophile granules are stained pink. Oxyphile granules, 

 brownish red. Basophile granules, purple. Nucleoli (plasmosomes), 

 pink. The cytoplasm of partially haemoglobinated precursors of 

 erythrocytes is stained in varying shades of reddish violet, while 

 mature erythrocytes are deep pinkish orange. 



References : 



Carleton, H. M. & Leach, E. H. (1947), p. 208. 

 Jenner, L. (1899). 



JENNER STAIN 



For cytological examination of blood 



Technique : 



1. Air-dried, unfixed blood films are stained for three minutes, 

 face downwards to prevent precipitate depositing on the film and 

 obscuring the picture. 



2. Wash with distilled water until the film appears pink to the 

 naked eye. 



3. Blot dry and examine. 



Results: 



Neutrophile granules: pink. Oxyphile granules: brownish red. 

 Basophile granules : purple. Nucleoli (plasmosomes) : pink. Cyto- 

 plasm of partially haemoglobinated precursors of erythrocytes: 

 varying shades of purple. Mature erythrocytes: deep pinkish 

 orange. 



Reference: Jenner, L. (1899). 



JENNER STAIN - GIEMSA STAIN 

 For the polychromatic staining of blood-forming organs 



Solutions required: 



A. Formol saline. 



B. Jenner Stain. 



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